mouse anti-bmprib  (Santa Cruz Biotechnology)

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Both the two cell types showed typical spindle-shaped morphology (×40) in regular cultures ( left ). After one week of osteogenic differentiation, a positive AP staining was detected in either dermal BMPRIB + cells or BMSCs ( middle ). Moreover, they were stained positive for AR after two weeks’ induction ( Right ). In contrast, only very week AP staining or AR staining was observed when they were cultured in non-osteogenic conditions (images not shown). BMPRIB + cells : morphogenetic protein receptor IB positive cells, BMSCs : bone marrow mesenchymal stromal cells, AP : alkaline phosphatase, AR : Alizarin Red.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Staining, Cell Culture

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Under osteogenic conditions, dermal BMPRIB + cells and BMSCs showed a similar AP production at one week (above , left) and calcium nodules formations at two week (above , right) . For osteogenic gene expression on mRNA level at day 14, dermal BMPRIB + cells produced a significantly higher OCN (a late osteogenic marker) expression than BMSCs, while transcript levels for Runx2 (an early osteogenic marker)and OPN (an intermediate osteogenic marker)were not significantly different between two groups (below) .Bars represent mean ± SD of n = 3 donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; BMSCs : bone marrow mesenchymal stromal cells; OPN : osteopontin; OCN : osteocalcin.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Gene Expression, Produced, Marker, Expressing

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Phenotypic analysis of C57BL/6 BMPRIB + cells and BMSCs before and after osteogenic differentiation by flow cytometry.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Cytometry

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Balb/c splenocytes were co-cultured with four mitotically inactivated different cell isolates at passage three at a cell ratio of 1:1for 5 days. Autologous mitotically inactivated splenocytes were set as negative control, while C57BL/6 mitotically inactivated splenocytes were set as positive control. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; BMSCs : bone marrow mesenchymal stromal cells; NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Cell Culture, Negative Control, Positive Control

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Four mitotically inactivated different cell isolates were added to allogeneic MLR at the numbers ranging from 10000, 50000, and 100000 cells per well. Autologous inactivated splenocytes were set as negative control. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; BMSCs : bone marrow mesenchymal stromal cells. NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Negative Control

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Following co-culture with allogeneic MLR for 5 days, undifferentiated BMPRIB + cells upregulated the iNOS expression drastically above the basal level, while IDO production was found to be upregulated greatly in osteogenic BMPRIB + cells. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; MLR : mixed lymphocyte reaction; iNOS : inducible NO synthase; IDO : indoleamine 2,3-dioxygenase; NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Co-Culture Assay, Expressing

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Mitotically inactivated undifferentiated C57BL/6 BMPRIB + cells (1×10 5 cells) were co-cultured with Responder Balb/c splenocytes and stimulator C57BL/6 splenocytes at a 1:1:1 ratio in 200μl culture medium. Cells were treated with 1400W (0.1mM, 1mM) or 1-MT (1mM) for 5 days and proliferation was performed respectively. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; 1400W : N-[[3-(aminomethyl)phenyl]methyl]-ethanimidamide; 1-MT :1-methyl tryptophan. NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Mitotically inactivated osteogenic differentiated C57BL/6 BMPRIB + cells (1×10 5 cells) were co-cultured with Responder Balb/c splenocytes and stimulator C57BL/6 splenocytes at a 1:1:1 ratio in 200μl culture medium. Cells were treated with 1-MT (0.1mM, 1mM) or 1400W (1mM) for 5 days and proliferation was performed respectively. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; 1400W : N-[[3-(aminomethyl)phenyl]methyl]-ethanimidamide; 1-MT : 1-methyl tryptophan. NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Cell Culture